Dishonest employees have allegedly fleeced more than $600,000 from a range of Alice Springs businesses.

Police said they had seen a disturbing trend where a large number of workers in the town had been duping their bosses of tens of thousands of dollars.

A 25-year-old woman who was employed by an Aboriginal art association allegedly took the most money when she managed to fraudulently transfer almost $250,000 out of the company accounts over an eight-month period.